Anti-PARP p85 Fragment pAb

Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair, is a well known substrate for caspase-3 cleavage during apoptosis. Anti-PARP p85 Fragment pAb is a rabbit polyclonal antibody specific for the p85 fragment of PARP that results from caspase cleavage of the 116kDa intact molecule and thus provides an in situ marker for apoptosis. The antibody is affinity-purified using a peptide that corresponds to a region of the p85 fragment of PARP. The PARP immunogen is a synthetic peptide, gly-val-asp-glu-val-ala-lys (GVDEVAK), representing the N-terminus of the large C-terminal fragment of human PAR...

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Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair, is a well known substrate for caspase-3 cleavage during apoptosis. Anti-PARP p85 Fragment pAb is a rabbit polyclonal antibody specific for the p85 fragment of PARP that results from caspase cleavage of the 116kDa intact molecule and thus provides an in situ marker for apoptosis. The antibody is affinity-purified using a peptide that corresponds to a region of the p85 fragment of PARP. The PARP immunogen is a synthetic peptide, gly-val-asp-glu-val-ala-lys (GVDEVAK), representing the N-terminus of the large C-terminal fragment of human PARP that results from caspase-3 cleavage. Each batch of antibody is quality assurance tested for use in immunostaining applications and contains sufficient antibody for 50 immunocytochemical reactions at the suggested working dilution of 1:100.

Features - Benefits

• Immunogen: N-terminal peptide from p85 fragment.
• Antibody Form: Affinity-purified rabbit polyclonal antibody provided in Dulbecco's PBS.
• Specificity: Specifically detects PARP p85 fragment in human, rat and bovine cells and tissues. Does not recognize the 116kDa intact PARP protein.

Applications

(Suggested working dilution)

• Western blotting (1:300 to 1:1,000).
• Immunocytochemistry (1:100).
• Immunohistochemistry (1:50).
• Flow cytometry.

For more information, see the Protocols & Applications Guide.

References

1. O'Brien, M.A., Moravec, R.A. and Riss, T.L. (2001) BioTechniques 30, 886–91.

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