Anti-ACTIVE® MAPK pAb, Rabbit, (pTEpY)

Anti-ACTIVE® MAPK pAb is a polyclonal rabbit antibody. The antibody is affinity purified using a dually phosphorylated peptide that corresponds to the active form of the mitogen-activated protein (MAP) kinase enzymes.

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Anti-ACTIVE® MAPK pAb is a polyclonal rabbit antibody. The antibody is affinity purified using a dually phosphorylated peptide that corresponds to the active form of the mitogen-activated protein (MAP) kinase enzymes.

Features - Benefits

• Specificity: Preferentially detects the dually phosphorylated, active form of the mitogen-activated protein kinase (MAPK) enzymes (ERK1 and ERK2).
• Immunogen: Dually phosphorylated Thr/Glu/Tyr region (pTEpY) derived from the catalytic core of the active form of the mitogen-activated protein kinase (MAPK) enzymes, ERK1 and ERK2, which corresponds to Thr¹⁸³ and Tyr¹⁸⁵ of the mammalian ERK2 enzyme.
• Antibody Form: Affinity-purified rabbit IgG; supplied in PBS (pH 7.4).
• Value: When used at the recommended 1:5,000 dilution, this product will generate 200ml of blotting solution, sufficient for 20 Western blots of 10ml each.

Applications

(Suggested working dilution)

• Western blotting (1:5,000).
• Immunocytochemistry (1:500).
• Immunohistochemistry (1:500).

For more information, see the Protocols & Applications Guide.

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