Bacterial Strain NM522

NM522 contains an F´ episome, which is required for production of ssDNA and for blue/white color selection. To maintain the F´, NM522 should be grown on minimal (M-9) medium.

Genotype: supE, thi, Δ(lac-proAB), hsd5 (r^–, m^–), [F´, proAB, lacI^qZΔM15].

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NM522 contains an F´ episome, which is required for production of ssDNA and for blue/white color selection. To maintain the F´, NM522 should be grown on minimal (M-9) medium.

Genotype: supE, thi, Δ(lac-proAB), hsd5 (r^–, m^–), [F´, proAB, lacI^qZΔM15].

Applications

• Production of ssDNA.

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