Promega Corporation

Alkaline Phosphatase, Calf Intestinal (CIAP)

Alkaline Phosphatase, Calf Intestinal (CIAP), catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vector DNA by removing phosphate groups from both 5´-termini and may also be used for the dephosphorylation of 5´ phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 Polynucleotide Kinase. CIAP is active on 5´ overhangs, 5´ recessed and blunt ends.

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Alkaline Phosphatase, Calf Intestinal

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Alkaline Phosphatase, Calf Intestinal

 Components

  • Alkaline Phosphatase, Calf Intestinal

    M182A1 x 1,000u
  • Alkaline Phosphatase 10X Buffer

    M183A2 x 500μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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1,000u
1u/μl M1821 C$ 78.00 Add to cart

Alkaline Phosphatase, Calf Intestinal (HC)

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Alkaline Phosphatase, Calf Intestinal (HC)

 Components

  • Alkaline Phosphatase 10X Buffer

    M183A2 x 500μl
  • Alkaline Phosphatase Calf Intestinal (HC)

    M282A1 x 1,000u
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000u
20u/μl M2825 C$ 78.00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

CIAP Buffer Pack

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CIAP Buffer Pack

 Components

  • Alkaline Phosphatase 10X Buffer

    M183A3 x 500μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1.5ml
(3 × 500μl)
- M1833 C$ 24.00 Add to cart

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QC Tests

Activity, endonuclease, DNase, RNase, blue/white cloning assay.

Source

Calf intestinal mucosa.

Storage Buffer

10mM Tris-HCl (pH 8.0), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1μmol of 4-nitrophenyl phosphate per minute at 37°C in 1M diethanolamine, 10.9mM 4-nitrophenyl phosphate, 0.5mM MgCl2 (pH 9.8 at 25°C).

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

M1821, M2825, M1833 For Research Use Only. Not for Use in Diagnostic Procedures.

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