DNA 5´ End-Labeling System

The DNA 5´ End-Labeling System is a complete system for phosphorylating both double- and single-stranded DNA and RNA with T4 Polynucleotide Kinase and [γ-³²P]ATP. The system includes enzymes, buffers and control DNA standards to measure reaction efficiencies. Calf Intestinal Alkaline Phosphatase is included for removal of the 5´-phosphate prior to labeling with T4 Polynucleotide Kinase.

Expand to Read More »

The DNA 5´ End-Labeling System is a complete system for phosphorylating both double- and single-stranded DNA and RNA with T4 Polynucleotide Kinase and [γ-³²P]ATP. The system includes enzymes, buffers and control DNA standards to measure reaction efficiencies. Calf Intestinal Alkaline Phosphatase is included for removal of the 5´-phosphate prior to labeling with T4 Polynucleotide Kinase.

Features - Benefits

• Convenient: Can use to label both single-stranded and double-stranded DNA and RNA.
• Complete: System includes enzymes, buffers and control DNA standards for measuring reaction efficiencies (except radionucleotides).
• Flexible: Works with [γ-³²P]ATP, [γ-³³P]ATP or [γ-³⁵S]ATP.

Applications

• Preparation of hybridization probes.
• Preparation of sequencing primers.
• Labeling marker DNAs and RNAs for autoradiography.
• Preparation of probes for gel shift assays.

For more information, see the Protocols & Applications Guide.

« Collapse Content