Promega Corporation

HQ and GST Tag Flexi® Vectors for E. coli and Cell-Free Protein Expression

The vectors below are used for inducible expression of HQ- and GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. The HQ tag and polyhistidine tag (His) are comparable in their affinity for Ni ions and will bind to all His-binding surfaces and resins. In certain cases the HQ-tagged proteins can be eluted from the affinity columns at lower concentrations of imidazole—a property useful for some downstream applications such as enzymatic reactions. As with His tag, proteins can be expressed from bacterial, insect and mammalian systems and purified under either native or...

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pFN2A (GST) Flexi® Vector

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pFN2A (GST) Flexi® Vector

 Components

  • pFN2A (GST) Flexi® Vector

    C846A1 x 20μg
20μg
- C8461 C$ 313.00 Add to cart

pFN2K (GST) Flexi® Vector

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pFN2K (GST) Flexi® Vector

 Components

  • pFN2K (GST) Flexi® Vector

    C847A1 x 20μg
20μg
- C8471 C$ 313.00 Add to cart

pFN6A (HQ) Flexi® Vector

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pFN6A (HQ) Flexi® Vector

 Components

  • pFN6A (HQ) Flexi® Vector

    C851A1 x 20μg
20μg
- C8511 C$ 313.00 Add to cart

pFN6K (HQ) Flexi® Vector

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pFN6K (HQ) Flexi® Vector

 Components

  • pFN6K (HQ) Flexi® Vector

    C852A1 x 20μg
20μg
- C8521 C$ 313.00 Add to cart

pFC7A (HQ) Flexi® Vector

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pFC7A (HQ) Flexi® Vector

 Components

  • pFC7A (HQ) Flexi® Vector

    C853A1 x 20μg
20μg
- C8531 C$ 313.00 Add to cart

pFC7K (HQ) Flexi® Vector

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pFC7K (HQ) Flexi® Vector

 Components

  • pFC7K (HQ) Flexi® Vector

    C854A1 x 20μg
20μg
- C8541 C$ 313.00 Add to cart

pF1A T7 Flexi® Vector

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pF1A T7 Flexi® Vector

 Components

  • pF1A T7 Flexi® Vector

    C844A1 x 20μg
20μg
- C8441 C$ 276.00 Add to cart

pF1K T7 Flexi® Vector

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pF1K T7 Flexi® Vector

 Components

  • pF1K T7 Flexi® Vector

    C845A1 x 20μg
20μg
- C8451 C$ 276.00 Add to cart


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GenBank®/EMBL Accession Number

pFN2A (GST) Accession Number AY753578; pFN2A (GST) Vector sequence text file.
pFN2K (GST) Accession Number AY753579; pFN2K (GST) Vector sequence text file.
pFN6A (HQ) Accession Number, DQ132629; pFN6A (HQ) Vector sequence text file.
pFN6K (HQ) Accession Number DQ132630; pFN6K (HQ) Vector sequence text file.
pFC7A (HQ) Accession Number DQ133906; pFC7A (HQ) Vector sequence text file.
pFC7K (HQ) Accession Number DQ133907; pFC7K (HQ) Vector sequence text file.
pF1A T7 Accession Number AY753576; pF1A T7 Vector sequence text file.
pF1K T7 Accession Number AY753577; pF1K T7 Vector sequence text file.

Storage Conditions

Store vectors at –20°C.

For product intended use please see Patents & Disclaimers tab.

pFN2A (GST) Flexi Vector.Figure 1. pFN2A (GST) Flexi® Vector.
pFN2K (GST) Flexi Vector.Figure 2. pFN2K (GST) Flexi® Vector.
pFN6A (HQ) Flexi Vector.Figure 3. pFN6A (HQ) Flexi® Vector.
pFN6K (HQ) Flexi Vector.Figure 4. pFN6K (HQ) Flexi® Vector.
pFC7A (HQ) Flexi Vector.Figure 5. pFC7A (HQ) Flexi® Vector.
pFC7K (HQ) Flexi Vector.Figure 6. pFC7K (HQ) Flexi® Vector.
pF1A T7 Flexi Vector.Figure 7. pF1A T7 Flexi® Vector.
pF1K T7 Flexi Vector.Figure 8. pF1K T7 Flexi® Vector.

Use Restrictions

C8461, C8471, C8511, C8521, C8531, C8541, C8441, C8451 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

C8461, C8471, C8511, C8521, C8531, C8541, C8441, C8451 European Pat. No. 1685247 and other patents pending.

C8461, C8471, C8511, C8521, C8531, C8541, C8441, C8451 Patent Pending.

C8461, C8471, C8511, C8521, C8531, C8541, C8441, C8451 Usage Restrictions for the T7 Expression System
The T7 expression system is based on technology developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy and is the subject of patents assigned to Brookhaven Science Associates, LLC (BSA). This technology, including bacteria, phage and plasmids that carry the gene for T7 RNA polymerase, is to be used for academic or nonprofit laboratory or licensed commercial research purposes only. By accepting or using the T7 expression technology you agree to be bound by the following conditions set forth by BSA. The initial purchaser may refuse to accept the conditions of this notice by returning this product and the enclosed materials to Promega unused.

Academic and NonProfit Laboratories
No materials that contain the cloned gene for T7 RNA polymerase may be distributed further to third parties outside of your laboratory unless the recipient receives a copy of this assurance notice and agrees to be bound by its terms. This limitation applies to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof.

Commercial Laboratories
A license is required for any commercial use of the T7 expression system, including use of the T7 system for research purposes or for production purposes by any commercial entity. Information about commercial licenses may be obtained from the Licensing Office, Brookhaven National Laboratory, Upton, NY 11973, Telephone: 631-344-7134, FAX: 631-344-3729.

C8461, C8471, C8511, C8521, C8531, C8541, C8441, C8451 Usage restrictions apply to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof.

C8461, C8471, C8511, C8521, C8531, C8541, C8441, C8451 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

C8461, C8471 This product or portions thereof is manufactured under license from Amrad Corporation Limited. For non-commercial research use only. All other uses require a license from Amrad Corporation Limited, Unit 6, 663 Victoria Street, Abbotsford, Victoria 3067, Australia, under U.S. Pat. No. 5,654,176, Australian Pat. No. 607511, Canadian Pat. No. 1338903 and other issued patents.

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