pGEM®-3Z Vector

The pGEM®-3Z Vector is intended for use as a standard cloning vector, as well as for the highly efficient synthesis of RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue/white screening. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.

The pGEM®-3Z and pGEM®-4Z Vectors are essentially identical except for the orientation of the SP6 and T7 promoters.

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The pGEM®-3Z Vector is intended for use as a standard cloning vector, as well as for the highly efficient synthesis of RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue/white screening. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.

The pGEM®-3Z and pGEM®-4Z Vectors are essentially identical except for the orientation of the SP6 and T7 promoters.

Features - Benefits

• Blue/White Screening: Allows the easy identification of recombinant clones.
• Versatile: This vector can be used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
• Convenient: Multiple cloning site provides a selection of restriction sites for cloning.

References

1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.

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