Single-Stranded DNA Binding Protein

E. coli Single-Stranded DNA Binding Protein (SSB) consists of four identical 18.9kDa subunits. It binds with high affinity in a cooperative manner to single-stranded DNA but does not bind well to double-stranded DNA. It is involved in DNA replication and in recombination in vivo.

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E. coli Single-Stranded DNA Binding Protein (SSB) consists of four identical 18.9kDa subunits. It binds with high affinity in a cooperative manner to single-stranded DNA but does not bind well to double-stranded DNA. It is involved in DNA replication and in recombination in vivo.

Applications

• Studying DNA replication and recombination.
• DNA sequencing using the primer walking strategy with contiguous oligonucleotides.

References

1. Sancar, A. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4274–8.
2. Chase, J.W. and Williams, K.R. (1986) Ann. Rev. Biochem. 55, 103–36.
3. Krauss, G. et al. (1981) Biochemistry 20, 5346–52.
4. Weiner, J.H. et al. (1975) J. Biol. Chem. 250, 1972–80.

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