Promega Corporation

QuantiFluor® RNA System

Sensitive quantitation of RNA is important for the success of downstream applications. The QuantiFluor® RNA System contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution. Detecting and quantitating small amounts of RNA is a very important step that is used in many biological applications, particularly in molecular biology techniques.

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QuantiFluor® RNA System

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QuantiFluor® RNA System

 Components

  • 20X TE Buffer (pH 7.5)

    E260A1 x 25ml
  • RNA Standard

    E299A1 x 100μg
  • QuantiFluor® RNA Dye

    E286A1 x 1ml
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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1ml
- E3310 C$ 273.00 Add to cart


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Storage Conditions

Store at –30°C to –10°C, protected from light.

For product intended use please see Patents & Disclaimers tab.

Figures

Standard curves using the QuantiFluor® RNA Dye and a competitor dye.Figure 1. Standard curves using the QuantiFluor® RNA Dye and a competitor dye.

The standard curves were generated using RNA Standard in a 96-well format and 200µl total volume as described in Section 5 of the Technical Manual. The standard curve RNA amounts are 2ng, 10ng, 20ng, 50ng, 100ng, 200ng and 500ng per well. Fluorescence was measured using the GloMax®-Multi+ Detection System. The fluorescence values shown were blank-subtracted. Under these conditions, the dynamic range for the QuantiFluor® RNA Dye is approximately 2–500ng per well (in 200µl total volume), and the QuantiFluor® RNA Dye limit of detection is approximately 100pg per well.

Use Restrictions

E3310 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

Patent Pending.

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