AccessQuick™ RT-PCR System

The AccessQuick™ RT-PCR System is an easy and convenient master mix system for setting up one-tube RT-PCR. It increases the convenience of performing RT-PCR by combining the following components in a single tube: Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer. The AMV RT enzyme is provided in a separate tube to allow important no-RT control reactions. The AccessQuick™ Master Mix is simply added to RNA templates in reaction vials, followed by the AMV RT, primers and water. The AccessQuick™ RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously op...

Expand to Read More »

The AccessQuick™ RT-PCR System is an easy and convenient master mix system for setting up one-tube RT-PCR. It increases the convenience of performing RT-PCR by combining the following components in a single tube: Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer. The AMV RT enzyme is provided in a separate tube to allow important no-RT control reactions. The AccessQuick™ Master Mix is simply added to RNA templates in reaction vials, followed by the AMV RT, primers and water. The AccessQuick™ RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously optimized and do not require extreme conditions.

Features - Benefits

• Maximum Convenience: Save yourself four pipetting steps. Simply combine the AccessQuick™ Master Mix, AMV RT, your gene-specific primers, your RNA template and water. Separate AMV RT allows important no-RT control reactions.
• Less Template: Amplify from zeptomole (10^–21mol) levels of RNA.
• No Buffer Additions Required: Set up reactions in a single tube, place in the thermal cycler, come back later for results—no additions between the reverse transcription and DNA amplification steps.
• Stability: System components are stable over many freeze-thaw cycles.

Applications

For more information, see the Protocols & Applications Guide.

« Collapse Content