CellTox™ Green Cytotoxicity Assay
Kinetic Cytotoxicity Determination Out to 72 Hours With Multiplexing Compatibility
Cytotoxicity assays based on detection of released biomarkers into the media can underestimate cytotoxicity in long term exposures of 72 hours or greater because of limited stability of the biomarker detected. The CellTox™ Green Cytotoxicity Assay provides an easy, fast and accurate method to determine toxic effects during or after long term exposure of cells in culture.
- Accurate cytotoxicity determination in exposures out to 72 hours
- Simple and flexible protocol allows kinetic analysis or endpoint determination of cytotoxicity
- Multiplex with luminescent assays to get more relevant data per well
- Easily scalable from 96- to 1536- well plate formats
Perform Kinetic Cytotoxicity Measurements out to 72 hours
CellTox™ Green Dye binds DNA of cells with impaired membrane integrity.
Easily Integrate the CellTox™ Green Assay Into Your Workflow
Multiplexing for More Informative Data Per Well
CellTox™ Green Dye was mixed with Hela Cells and plated, then dosed with compound. CellTiter-Glo® Cell Viability Assay Reagent was added at the end of the 72 hour exposure and luminescence (viability) measured. The inverse measures of cell health resulted in EC50 agreement.
Kinetic Cytotoxicity Measures to Determine Mechanism of Toxicity
CellTox™ Green Dye can be used in kinetic mode to determine the mechanism of toxicity. Panel A: A full time course of repeated measurements at 4, 24, 48 and 72 hours demonstrates the onset of cytotoxicity. Panel B: At the first indication of cytotoxicity, a time course experiment was interrupted at 24 hours by addition of Caspase-Glo® 3/7 Assay reagent (caspase activation, luminescence), indicating cytotoxicity is the result of apoptosis. Had caspase activation been measured at 72 hours, the caspase activation event may have been underestimated or missed due to enzymatic degradation of the caspase enzyme over time.