pCMVTnT™ and pTnT™ Vectors

The pCMVTnT™ and pTnT™ Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems. The presence of RNA phage promoters also allows highly efficient synthesis of RNA in vitro. Both vectors contain a 5´ β-globin leader sequence and synthetic poly(A)30 tail, which have been shown to enhance expression of certain genes.

For in vivo expression, the pCMVTnT™ Vector contains a CMV enhancer/promoter region, which allows strong constitutive expression in many cell...

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The pCMVTnT™ and pTnT™ Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems. The presence of RNA phage promoters also allows highly efficient synthesis of RNA in vitro. Both vectors contain a 5´ β-globin leader sequence and synthetic poly(A)30 tail, which have been shown to enhance expression of certain genes.

For in vivo expression, the pCMVTnT™ Vector contains a CMV enhancer/promoter region, which allows strong constitutive expression in many cell types.

Features - Benefits

• Flexible: Tandem SP6 and T7 phage promoters allow use in the appropriate in vitro translation or transcription system.
• Convenient: Multiple cloning site provides a selection of restriction sites.
• In Vivo Expression: The CMV enhancer/promoter region in the pCMVTnT™ Vector allows strong constitutive expression in many cell types.

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