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E. coli S30 Extract System for Circular DNA

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The E. coli S30 Extract for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or λ vectors, providing a powerful tool for identifying and characterizing polypeptides. The investigator needs only to supply the cloned DNA containing the appropriate prokaryotic promoter and ribosome binding sites. The S30 Extract for Circular DNA Templates is prepared by modifications of the method described by Zubay from an E. coli strain B deficient in OmpT endoproteinase and lon protease activity. This results in a greater stability of expressed proteins that would otherwise be degraded by pr...

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E. coli S30 Extract System for Circular DNA

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E. coli S30 Extract System for Circular DNA

 Components

  • Amino Acid Mixture Minus Cysteine

    L447A1 x 175μl
  • S30 Extract, Circular

    L464A3 x 150μl
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • Luciferase Dilution Reagent

    L487A1 x 1ml
  • pBESTluc™ DNA, Circular (Control)

    L492A1 x 10μg
  • S30 Premix without Amino Acids

    L512A1 x 750μl
  • Amino Acid Mixture Minus Leucine

    L995B1 x 175μl
  • Amino Acid Mixture Minus Methionine

    L996B1 x 175μl
30 reactions
- L1020 C$ 443.00 Add to cart


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Storage Conditions

Store at –70°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

L1020 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

L1020 U.S. Pat. Nos. 5,641,641 and 5,650,289.

L1020 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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