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E. coli S30 Extract System for Linear Templates

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The E. coli S30 Extract System for Linear Templates is prepared using minor modifications of the protocol described by Lesley and colleagues and allows successful transcription/translation of linear DNA templates. The investigator need only provide linear DNA containing a prokaryotic E. coli-like promoter (such as lacUV5, tac, λPL (con) and λ-PR). A ribosome binding site is required to direct the synthesis of proteins in vitro. In vitro-generated RNA from DNA templates lacking an E. coli promoter may also be used in this system, but protein yields will be decreased to 1–10% of that produced from linear DNA templat...

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E. coli S30 Extract System for Linear Templates

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E. coli S30 Extract System for Linear Templates

 Components

  • Amino Acid Mixture Minus Cysteine

    L447A1 x 175μl
  • S30 Extract, Linear

    L480A3 x 150μl
  • pBESTluc™ DNA, Linear (Control)

    L485A1 x 10μg
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • Luciferase Dilution Reagent

    L487A1 x 1ml
  • S30 Premix without Amino Acids

    L512A1 x 750μl
  • Amino Acid Mixture Minus Leucine

    L995B1 x 175μl
  • Amino Acid Mixture Minus Methionine

    L996B1 x 175μl
30 reactions
- L1030 C$ 443.00 Add to cart


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Storage Conditions

Store at –70°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

L1030 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

L1030 U.S. Pat. Nos. 5,641,641 and 5,650,289.

L1030 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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