S30 T7 High-Yield Protein Expression System

The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site. The protein expression system provides an extract that contains T7 RNA polymerase for transcription and is deficient in OmpT endoproteinase and lon protease activity. All other necessary components in the system are optimized for protein expression. This results in greater stability and enhanced expression of target proteins.

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The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site. The protein expression system provides an extract that contains T7 RNA polymerase for transcription and is deficient in OmpT endoproteinase and lon protease activity. All other necessary components in the system are optimized for protein expression. This results in greater stability and enhanced expression of target proteins.

Features - Benefits

• Obtain Data Faster: Protein expression in only one hour, not days as with cell-based expression.
• Complete System: No requirement to purchase additional reagents.
• Achieve High Protein Expression: Express up to 500μg/ml of protein for multiple applications.
• Scalable: Convenient screening protocol for high-throughput protein expression.
• Flexible: Detect expressed proteins by Coomassie® staining or incorporation of a fluorescence or biotinylated modified tRNA.

Applications

• Protein:protein interactions.
• Protein:DNA interactions.
• Enzyme activity.
• Antibody production.

For more information, see the Protocols & Applications Guide.

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