Promega Corporation

HaloTag® Fusion (N-Terminal) Mammalian Expression Vectors

See All HaloTag® Vectors
HaloTag® Fusion (C-Terminal) Mammalian Expression Vectors
Inducible T7-Driven Flexi® Vectors for E. coli Expression

These vectors are designed for expression of N-terminal-tagged HaloTag® fusion proteins in mammalian cells. Once expressed, the HaloTag® fusion protein may be used for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag® Ligands. In addition, the HaloTag® fusion protein can be purified or pulled down as a complex with its protein partners.

We offer two types of HaloTag® fusion ve...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

pHTN HaloTag® CMV-neo Vector

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pHTN HaloTag® CMV-neo Vector

 Components

  • pHTN HaloTag® CMV-neo Vector

    G772A1 x 20μg
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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20μg
- G7721 C$ 236.00 Add to cart

pFN28A HaloTag® CMV-neo Flexi® Vector

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pFN28A HaloTag® CMV-neo Flexi® Vector

 Components

  • pFN28A HaloTag® CMV-neo Flexi® Vector

    G844A1 x 20μg
20μg
- G8441 C$ 258.00 Add to cart

pFN28K HaloTag® CMV-neo Flexi® Vector

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pFN28K HaloTag® CMV-neo Flexi® Vector

 Components

  • pFN28K HaloTag® CMV-neo Flexi® Vector

    G845A1 x 20μg
20μg
- G8451 C$ 258.00 Add to cart

pFN21A HaloTag® CMV Flexi® Vector

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pFN21A HaloTag® CMV Flexi® Vector

 Components

  • pFN21A HaloTag® CMV Flexi® Vector

    G282A1 x 20μg
20μg
- G2821 C$ 258.00 Add to cart

pFN21K HaloTag® CMV Flexi® Vector

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pFN21K HaloTag® CMV Flexi® Vector

 Components

  • pFN21K HaloTag® CMV Flexi® Vector

    G283A1 x 20μg
20μg
- G2831 C$ 258.00 Add to cart

pFN22A HaloTag® CMVd1 Flexi® Vector

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pFN22A HaloTag® CMVd1 Flexi® Vector

 Components

  • pFN22A HaloTag® CMVd1 Flexi® Vector

    G284A1 x 20μg
20μg
- G2841 C$ 258.00 Add to cart

pFN22K HaloTag® CMVd1 Flexi® Vector

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pFN22K HaloTag® CMVd1 Flexi® Vector

 Components

  • pFN22K HaloTag® CMVd1 Flexi® Vector

    G285A1 x 20μg
20μg
- G2851 C$ 258.00 Add to cart

pFN23A HaloTag® CMVd2 Flexi® Vector

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pFN23A HaloTag® CMVd2 Flexi® Vector

 Components

  • pFN23A HaloTag® CMVd2 Flexi® Vector

    G286A1 x 20μg
20μg
- G2861 C$ 258.00 Add to cart

pFN23K HaloTag® CMVd2 Flexi® Vector

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pFN23K HaloTag® CMVd2 Flexi® Vector

 Components

  • pFN23K HaloTag® CMVd2 Flexi® Vector

    G287A1 x 20μg
20μg
- G2871 C$ 258.00 Add to cart

pFN24A HaloTag® CMVd3 Flexi® Vector

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pFN24A HaloTag® CMVd3 Flexi® Vector

 Components

  • pFN24A HaloTag® CMVd3 Flexi® Vector

    G288A1 x 20μg
20μg
- G2881 C$ 258.00 Add to cart

pFN24K HaloTag® CMVd3 Flexi® Vector

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pFN24K HaloTag® CMVd3 Flexi® Vector

 Components

  • pFN24K HaloTag® CMVd3 Flexi® Vector

    G298A1 x 20μg
20μg
- G2981 C$ 258.00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

HaloTag® Cloning Starter System

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HaloTag® Cloning Starter System

 Components

  • Wizard® SV Gel and PCR Clean-Up System

    A92801 x 10 preps
  • Flexi® System, Entry/Transfer

    C86411 x 1 each
  • HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack

    G37801 x 9 × 2μg
  • Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

    R19011 x 50μl
1 each
- G6050 C$ 378.00 Add to cart

HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack

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HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack

 Components

  • pFC17K HaloTag® CMVd3 Flexi® Vector

    G132B1 x 2μg
  • pFC16K HaloTag® CMVd2 Flexi® Vector

    G157B1 x 2μg
  • pFC15K HaloTag® CMVd1 Flexi® Vector

    G160B1 x 2μg
  • pFN21A HaloTag® CMV Flexi® Vector

    G282B1 x 2μg
  • pFN21K HaloTag® CMV Flexi® Vector

    G283B1 x 2μg
  • pFN22K HaloTag® CMVd1 Flexi® Vector

    G285B1 x 2μg
  • pFN23K HaloTag® CMVd2 Flexi® Vector

    G287B1 x 2μg
  • pFN24K HaloTag® CMVd3 Flexi® Vector

    G298B1 x 2μg
  • pFC14K HaloTag® CMV Flexi® Vector

    G966B1 x 2μg
9 × 2μg
- G3780 C$ 258.00 Add to cart

You Also May Be Interested In

GenBank®/EMBL Accession Number

pHTN HaloTag® CMV-neo Accession Number JF920304; pHTN HaloTag® CMV-neo Vector sequence text file.
pFN28A HaloTag® CMV-neo Accession Number JN129497; pFN28A HaloTag® CMV-neo Vector sequence text file.
pFN28K HaloTag® CMV-neo Accession Number JN129498; pFN28K HaloTag® CMV-neo Vector sequence text file.
pFN21A HaloTag® CMV Accession Number EU621374; pFN21A HaloTag® CMV Vector sequence text file.
pFN21K HaloTag® CMV Accession Number EU621375; pFN21K HaloTag® CMV Vector sequence text file.
pFN22A HaloTag® CMVd1 Accession Number EU621376; pFN22A HaloTag® CMVd1 Vector sequence text file.
pFN22K HaloTag® CMVd1 Accession Number EU621377; pFN22K HaloTag® CMVd1 Vector sequence text file.
pFN23A HaloTag® CMVd2 Accession Number EU621378; pFN23A HaloTag® CMVd2 Vector sequence text file.
pFN23K HaloTag® CMVd2 Accession Number EU621379; pFN23K HaloTag® CMVd2 Vector sequence text file.
pFN24A HaloTag® CMVd3 Accession Number EU621380; pFN24A HaloTag® CMVd3 Vector sequence text file.
pFN24K HaloTag® CMVd3 Accession Number EU621381; pFN24K HaloTag® CMVd3 Vector sequence text file.

Storage Conditions

Store vectors at –20°C.

For product intended use please see Patents & Disclaimers tab.

pHTN HaloTag® CMV-neo VectorFigure 1. pHTN HaloTag® CMV-neo Vector
pFN28A HaloTag® CMV-neo Flexi® VectorFigure 2. pFN28A HaloTag® CMV-neo Flexi® Vector
pFN28K HaloTag® CMV-neo Flexi® VectorFigure 3. pFN28K HaloTag® CMV-neo Flexi® Vector
pFN21A HaloTag CMV Flexi Vector 7549MAFigure 4. pFN21A HaloTag® CMV Flexi® Vector.
pFN21A HaloTag CMV Flexi Vector 7550MAFigure 5. pFN21K HaloTag® CMV Flexi® Vector.
pFN22A HaloTag CMVd1 Flexi Vector 7551MAFigure 6. pFN22A HaloTag® CMVd1 Flexi® Vector.
pFN22K HaloTag CMVd1 Flexi Vector 7552MAFigure 7. pFN22K HaloTag® CMVd1 Flexi® Vector.
pFN23A HaloTag CMVd2 Flexi Vector 7553MAFigure 8. pFN23A HaloTag® CMVd2 Flexi® Vector.
pFN23K HaloTag CMVd2 Flexi Vector 7554MAFigure 9. pFN23K HaloTag® CMVd2 Flexi® Vector.
pFN24A HaloTag CMVd3 Flexi Vector 7555MAFigure 10. pFN24A HaloTag® CMVd3 Flexi® Vector.
pFN24K HaloTag CMVd3 Flexi Vector 7556MAFigure 11. pFN24K HaloTag® CMVd3 Flexi® Vector.
Relative mammalian protein expression levels for HaloTag Flexi Vectors.Table 1. Relative Mammalian Protein Expression Levels for HaloTag® Flexi® Vectors.

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HaloTag Flexi Vectors.Table 2. HaloTag® Flexi® Vectors.

Use Restrictions

G7721, G8441, G8451, G2821, G2831, G2841, G2851, G2861, G2871, G2881, G2981, G6050, G3780 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G7721, G8441, G8451, G2821, G2831, G2841, G2851, G2861, G2871, G2881, G2981, G6050, G3780 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

G7721, G8441, G8451, G2821, G2831, G2841, G2851, G2861, G2871, G2881, G2981, G6050, G3780 U.S. Pat. Nos. 7,425,436 and 7,935,803 and other patents pending.

G8441, G8451, G2821, G2831, G2841, G2851, G2861, G2871, G2881, G2981, G6050, G3780 European Pat. No. 1685247 and other patents pending.

G6050, G3780 Patent Pending.

G6050 U.S. Pat. No. 5,391,487.

G8441, G8451, G2821, G2831, G2841, G2851, G2861, G2871, G2881, G2981, G6050, G3780 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

G6050 Licensed under U.S. Pat. No. 5,945,288.

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