Choose the system that best meets your recombinant protein capture needs.
HaloTag® Purification from Mammalian Cells
This system offers strong improvements in recovery, purity, cost and protein functionality over FLAG® and polyhistidine tag purification strategies.
HaloTag® Purification from E. coli
A highly efficient system for soluble, tag-free purified protein—even when expressed at low levels.
HaloTag® Pull-Down for Protein:Protein Interactions
Capture novel in vivo protein interactions and complexes with ultra-low background.
HaloTag® Pull-Down for Protein:DNA Interactions
No antibody required! Identify protein:DNA interactions in 24-48 hours with a simple, proven ChIP system.
HisLink™ Protein Purification Systems
Efficient capture of polyhistidine or HQ-tagged proteins directly from cell lysates. Adaptable for use in manual or automated formats. Choose from spin, vacuum or magnetic-based systems.
Biotinylated Column and Batch Purification
A complete system for purifying in vivo biotinylated proteins under gentle, non-denaturing conditions.
A protocol that provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels.
This article describes how purification using HaloTag® technology gives high protein yield and purity, with no protein tag carryover.
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
Ask us! We are here to help you.