HaloTag® technology offers enhanced yield and solubility, rapid-purification of tag-free protein, and easy protein detection and quantitation.
HaloTag® Purification from Mammalian Cells
This system offers strong improvements in recovery, purity, cost and protein functionality over FLAG® and polyhistidine tag purification strategies.
HaloTag® Purification from E. coli
A highly efficient system for soluble, tag-free purified protein—even when expressed at low levels.
HaloTag® Pull-Down for Protein:Protein Interactions
Capture novel in vivo protein interactions and complexes with ultra-low background.
HaloTag® Pull-Down for Protein:DNA Interactions
No antibody required! Identify protein:DNA interactions in 24-48 hours with a simple, proven ChIP system.
HaloTEV Protease
HaloTEV Protease binds covalently to HaloLink™ Resin, providing convenient protein cleavage and a cleaner purified protein.
Experimentally Validated ORF/cDNAs
Start with an ORF/cDNA clone that is already functionally tested. Sequence is never enough!
This article compares the purity and yield of proteins purified using the HaloTag® Mammalian Protein Purification and Detection Systems to those from other commonly used protein methods.
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
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