HaloTEV Protease

HaloTEV Protease (81kDa) is a fusion between the HaloTag® protein and TEV protease, a highly specific proteolytic enzyme that cleaves at a specific TEV site, a specific seven-amino-acid sequence (ENLYFQ(G/S)). HaloTEV Protease allows covalent immobilization on HaloLink™ Resin and removal from a cleavage reaction in a single-step purification. The covalent capture of HaloTEV Protease improves purity of the final target protein and assures the improved recovery of the TEV protease.

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HaloTEV Protease (81kDa) is a fusion between the HaloTag® protein and TEV protease, a highly specific proteolytic enzyme that cleaves at a specific TEV site, a specific seven-amino-acid sequence (ENLYFQ(G/S)). HaloTEV Protease allows covalent immobilization on HaloLink™ Resin and removal from a cleavage reaction in a single-step purification. The covalent capture of HaloTEV Protease improves purity of the final target protein and assures the improved recovery of the TEV protease.

Features - Benefits

• Improve Final Protein Purity: Covalently remove HaloTEV from your purified protein with HaloLink™ Resin.
• Optimized for HaloTag® Purifications: Proteins can be purified tag-free in a single step as the HaloLink™ Resin will bind both HaloTag® protein tag and the HaloTEV protease.

References

1. Ryu, M.-J. et al. (2012) Oncogenic KRas expression in postmitotic neurons leads to S100A8-S100A9 protein overexpression and gliosis. J. Biol. Chem. 287, 22948–58.
2. Ohana, R.F. et al. (2011) HaloTag-based purification of functional human kinases from mammalian cells. Protein Expr. Purif. 76, 154–64.

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