Promega Corporation

Nano-Glo® Luciferase Assay System

The Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media. The reagent is prepared by mixing Nano-Glo® Luciferase Assay Substrate and Nano-Glo® Luciferase Assay Buffer. The reagent contains an integral lysis buffer allowing use directly on cells expressing NanoLuc® luciferase or the culture media when luciferase is secreted.

Nano-Glo® Luciferase Assay Reagent is a dedicated product for the detection of NanoLuc® L...

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Nano-Glo® Luciferase Assay
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Nano-Glo® Luciferase Assay  Components

  • Nano-Glo® Luciferase Assay Buffer

    N112A1 x 10ml
  • Nano-Glo® Luciferase Assay Substrate

    N113A1 x 200μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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10ml
- N1110 C$ 158.00 Add to cart
Nano-Glo® Luciferase Assay
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Nano-Glo® Luciferase Assay  Components

  • Nano-Glo® Luciferase Assay Buffer

    N112B1 x 100ml
  • Nano-Glo® Luciferase Assay Substrate

    N113B2 x 1ml
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100ml
- N1120 C$ 896.00 Add to cart
Nano-Glo® Luciferase Assay
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Nano-Glo® Luciferase Assay  Components

  • Nano-Glo® Luciferase Assay Buffer

    N112A10 x 10ml
  • Nano-Glo® Luciferase Assay Substrate

    N113A10 x 200μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10 × 10ml
- N1130 C$ 1,075.00 Add to cart
Nano-Glo® Luciferase Assay
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Nano-Glo® Luciferase Assay  Components

  • Nano-Glo® Luciferase Assay Buffer

    N112B10 x 100ml
  • Nano-Glo® Luciferase Assay Substrate

    N113C5 x 4ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10 × 100ml
- N1150 C$ 7,503.00 Add to cart


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Storage Buffer

Store at –20°C. Please refer to the Technical Manual for other short-term storage options.

For product intended use please see Patents & Disclaimers tab.

A comparison of the sensitivity of NanoLuc®, firefly and Renilla luciferase assays.Figure 1. A comparison of the sensitivity of NanoLuc®, firefly and Renilla luciferase assays.
Signal decay kinetics for Nano-Glo® Luciferase Assay Reagent.Figure 2. Signal decay kinetics for Nano-Glo® Luciferase Assay Reagent.
The bioluminescent reaction catalyzed by NanoLuc® luciferase.Figure 3. The bioluminescent reaction catalyzed by NanoLuc® luciferase.

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Use Restrictions

N1110, N1120, N1130, N1150 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

N1110, N1120, N1130, N1150 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researchers may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researchers shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

In addition, researchers must:
(1a) use NanoLuc®-branded luciferase or derivatives, subject to the NanoLuc® Limited Use Label License, for all luciferase assays conducted with this product; or
(1b) contact Promega to obtain a license for use of this product with alternative luciferases.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET™-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(2b) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

N1110, N1120, N1130, N1150 Patent Pending.

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