The T7 RiboMAX™ Express Large Scale RNA Production System is an in vitro transcription system designed for the consistent production of milligram amounts of RNA in a short amount of time. Due to optimization of the enzyme mix and transcription buffer, yields of 5–8.5mg/ml are generated in 30 minutes, compared to 2–4 hours with other commercially available systems. To minimize pipetting steps and errors, the 2X transcription buffer includes all four rNTPs. In addition, the system includes RQ1 RNase-Free DNase for the removal of plasmid template after transcription.
Due to the combined 2X buffer and rNTPs, the ...
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Due to the combined 2X buffer and rNTPs, the T7 RiboMAX™ Express System is not recommended for the synthesis of RNA for applications that require capped RNA. For synthesis of capped RNA, please order the standard RiboMAX™ Large Scale RNA Production System—T7 (Cat.# P1300).
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T7 RiboMAX™ Express Large Scale RNA Production System
RQ1 RNase-Free DNase
Enzyme Mix, T7 Express
Sodium Acetate, 3.0M (pH 5.2)
RiboMAX™ Express T7 2X Buffer
pGEM® Express Positive Control Template
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Store at –20°C.
For product intended use please see Patents & Disclaimers tab.
P1320 For Research Use Only. Not for Use in Diagnostic Procedures.
U.S. Pat. No. 5,552,302.
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