Suppress protein expression transiently or permanently using tools designed for producing high efficiency RNAi responses. Produce milligram amounts of double-stranded RNA (dsRNA) suitable for RNAi in both mammalian and nonmammalian systems.
psiCHECK™ Vectors
Rapidly screen RNAi by quantifying the Renilla luciferase reporter gene activity. Avoid labor-intensive, time-consuming assays and waiting for phenotypic changes.
T7 RiboMAX™ Express RNAi System
Generate milligram amounts of protein-free dsRNA in less than 30 minutes. The dsRNA is suitable for RNAi in both mammalian and nonmammalian systems.
In this article, we present data demonstrating the utility of the psiCHECK™-2 Vector as a biosensor for microRNA activity in cells.
This chapter of the Protocols and Applications Guide discusses the design and optimization of siRNA sequences, interference optimization, and delivery strategies for siRNA.
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