Perform functional analysis of embryonic stem cells and induced pluripotent stem cells using protein expression, gene knockdown and gene regulation tools. Uncover gene activity affecting stem cell differentiation and pluripotency.
pCI Mammalian Expression Vector
The pCI Vector's CMV enhancer/promoter region enables strong, constitutive expression in many cell types. A β-globin/IgG chimeric intron located downstream of the enhancer/promoter region can further increase expression.
pGL4 In Vivo Imaging Vectors
Experience improved sensitivity with increased reporter gene expression levels. The optimized vector backbone produces fewer artifacts with offers a choice of luc2 or hRluc detection.
FuGENE® HD Transfection Reagent
Transfect DNA into a wide variety of cell lines with high efficiency and low toxicity! There is no need to remove serum or culture medium after introducing the reagent:DNA complex.
HaloTag® Flexi Vectors
Vectors for directional cloning of protein-coding sequences to create HaloTag® fusion proteins for use in imaging and other applications.
In thie article, we covalently tether different wavelength fluorophores to a specially designed reporter, the HaloTag® protein, express it in human neural stem cells (hNSC), and label it with HaloTag® Ligands.
This animation shows how we exploit the chemistry of the bioluminescent reaction to create a suite of robust and sensitive biological assays.
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
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