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Caspase-Glo® 3/7 Assay: Use Fewer Cells and Spend Less Time with this Homogeneous Assay

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Abstract

The newly developed Caspase-Glo® 3/7 Assay allows researchers to detect caspase-3 and -7 activity in as few as 20 anti-Fas mAb-treated Jurkat cells. This luminescent assay is designed for multiwell-plate formats, and because it requires no washing or removal of cell culture medium, it is ideal for high-throughput screening applications. The assay demonstrates excellent Z´-factor values, indicating a robust assay.

Cell Notes 6, 13–15.

Martha O'Brien, Richard Moravec and Terry Riss

Promega Corporation
Publication Date: 2003

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