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High-Throughput Screening Using a Universal Luminescent Kinase Assay

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Abstract

Phosphotransferases represent a major group of cellular enzymes that use ATP as a substrate and are implicated in a wide variety of cellular functions. The homogeneous Kinase-Glo® Luminescent Kinase Assay can monitor the activity of these enzymes, which have become important drug discovery targets. This non-radioactive assay is robust and easily amenable to high-throughput screening applications.

Cell Notes 10, 20–23.

Said Goueli1, Brad Larson1, Kevin Hsiao1, Tracy Worzella1, Aoife Gallagher2, and Eric Matthews3

1Promega Corporation, 2Deerac Fludics, 3BMG LABTECH 

Publication Date: 2004

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