Ribonucleases (RNases) are ubiquitous enzymes that can detrimentally affect RNA isolation in the laboratory. Many applications such as RT-PCR and in vitro transcription and translation may be affected as well. RNase can renature after autoclaving and is difficult to inactivate. Many RNase inhibitors are available commercially. Promega's RNasin® Ribonuclease Inhibitor, one of the first introduced, is exceptionally effective at controlling RNase activity during molecular biology procedures.
Promega Notes 77, 8–11.
John Shultz, Robin Hurst and Natalie Betz