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Technically Speaking: Subcloning Plasmid DNA Constructs

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Abstract

One of the most common applications in the field of molecular biology is the generation of recombinant plasmids for gene expression. However, this seemingly trivial task requires a number of considerations. Here we examine parameters related to the optimization of cloning and screening for plasmid constructs.

Promega Notes 77, 28–29.

Bob McLaren

Promega Corporation
Publication Date: 2001
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