This article illustrates tissue staining with Promega polyclonal antibodies in both mouse and rat systems. We show immunohistochemical data for Anti-ACTIVE® MAPK, Anti-ACTIVE® JNK, Anti-ACTIVE® p38 and Anti-ACTIVE® CaM KII pAbs. In addition, we present work using polyclonal antibodies for the study of apoptosis (Anti-PARP p85 Fragment pAb, Anti-ACTIVE® Caspase-3 pAb, and Anti-pS473 Akt pAb). Protocols for immunohistochemical data are provided.
Promega Notes 80, 20–23.
Randy Hoffman1, Martha O'Brien1, and Bruce W. Jarvis2