Automated fluorescent sequencing using energy transfer dyes is the current method of choice for DNA sequence determination. If not removed prior to analysis, certain reaction components such as buffer salts and unincorporated dye-labeled terminators can interfere with data collection in a capillary-based automated sequencer. Likewise, the presence of contaminants such as salts, unincorporated dNTPs and primers can interfere with analysis of PCR fragments by capillary electrophoresis or microarray spotting. Promega has developed high-throughput, validated, walkaway systems to remove these contaminants using the Beckman Coulter Biomek® FX liquid handler and the flexible format of the MagneSil® Paramagnetic Particles.
Promega Notes 85, 3–6.
Brad Larson and Tom Strader