The Chroma-Luc™ Reporter Vectors encode luciferases that emit green and red luminescence. Although the luciferases are 98% identical in amino acid sequence, their peak luminescence wavelengths are separated by >75nm. Both colors of luminescence are generated by a single addition of Chroma-Glo™ Reagent, which has been developed for homogeneous assay of mammalian cells directly in culture medium. These novel vectors are useful for dual measurements where closely similar reporter structures are preferred or where a single reagent addition is desired.
Promega Notes 85, 11–14.
Brian Almond, Erika Hawkins, Pete Stecha, Denise Garvin, Aileen Paguio, Braeden Butler, Michael Beck, Monika Wood and Keith Wood