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The T7 RiboMAX™ Express RNAi System: Efficient Synthesis of dsRNA for RNA Interference

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Abstract

The T7 RiboMAX™ Express RNAi System is an in vitro transcription system designed for the efficient synthesis of milligram amounts RNA in as little as 30 minutes. The RNA is then used to generate double-stranded RNA (dsRNA), which can be used for RNA interference (RNAi) assays in nonmammalian systems. The system is quick, flexible and easy to use. In this article, we show that dsRNA generated using the T7 RiboMAX™ Express RNAi System is functional for RNAi using a Drosophila S2 cell model.

Promega Notes 84, 7–11.

Natalie Betz and Tracy Worzella

Promega Corporation
Publication Date: 2003

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