Promega Corporation

Purification of GST Fusion Proteins by Magnetic Resin-Based MagneGST™ Particles

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Abstract

The MagneGST™ Protein Purification System provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins with high efficiency and low background. The magnetic resin-based batch purification method eliminates the need for multiple centrifugation cycles and provides a simple purification that can be performed manually or on a robotic platform. Purification of the GST-fusion protein starting from cell pellets can be accomplished in less than 2 hours from either crude or cleared lysates.

Promega Notes 86, 6–10.

Marjeta Urh, Don Creswell, Jacqui Sankbeil, Dan Simpson, Rod Flemming, Cris Cowan and Gary Kobs

Promega Corporation
Publication Date: 2004

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