We describe the Regulated Mammalian Expression System, which allows researchers to achieve tightly controlled protein expression in mammalian cells. This system uses coumerin-based compounds to modulate regulation of gene expression while maintaining high levels of induction over low levels of basal activity. In addition, expression of the gene of interest can be turned off rapidly by novobiocin addition. Introduction of a unique auto-amplification loop for transactivator expression makes the system robust and minimizes potential undesired effects on host gene expression.
Promega Notes 95, 8–11.
Jolanta Vidugiriene, Jim Hartnett, Elaine Schenborn, and Michael R. Slater