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Identify P-glycoprotein Substrates and Inhibitors with the Rapid HTS Pgp-Glo™ Assay System

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Abstract

The Pgp-Glo™ Assay Systems are luminescent P-glycoprotein (Pgp) ATPase assays. The Pgp-Glo™ Assay detects the effects of compounds on recombinant human Pgp in a cell membrane fraction. The assay couples the ATPase activity of Pgp to the ATP dependence of the light-generating reaction of firefly luciferase. The rate of ATP consumption by Pgp is inversely correlated with the light output of the luciferase reaction. The Pgp-Glo™ Assay is easily amenable to high-throughput screening and can complement cell-based Pgp studies.

Promega Notes 96, 11–14.

Dongping Ma and James J. Cali
Promega Corporation
 Publication Date: 2007

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Identify P glycoprotein Substrates and Inhibitors with the Rapid HTS Pgp Glo Assay System 4 5 1 1
Hi Dongping Ma and James J. Cali, I'am wondering if you please publish the Pgp ATPase activity EC50s of compounds shown in Figure 4. Thank you. Best Regards, CK 29. November 2012
1-1 von 1
Identify P glycoprotein Substrates and Inhibitors with the Rapid HTS Pgp Glo Assay System 4 5 1 1
Hi Dongping Ma and James J. Cali, I'am wondering if you please publish the Pgp ATPase activity EC50s of compounds shown in Figure 4. Thank you. Best Regards, CK November 29, 2012
1-1 of 1
Identify P glycoprotein Substrates and Inhibitors with the Rapid HTS Pgp Glo Assay System 4 5 1 1
Hi Dongping Ma and James J. Cali, I'am wondering if you please publish the Pgp ATPase activity EC50s of compounds shown in Figure 4. Thank you. Best Regards, CK 29 de noviembre de 2012
1-1 de 1
Identify P glycoprotein Substrates and Inhibitors with the Rapid HTS Pgp Glo Assay System 4 5 1 1
Hi Dongping Ma and James J. Cali, I'am wondering if you please publish the Pgp ATPase activity EC50s of compounds shown in Figure 4. Thank you. Best Regards, CK 29 novembre 2012
1-1 sur 1
Identify P glycoprotein Substrates and Inhibitors with the Rapid HTS Pgp Glo Assay System 4 5 1 1
Hi Dongping Ma and James J. Cali, I'am wondering if you please publish the Pgp ATPase activity EC50s of compounds shown in Figure 4. Thank you. Best Regards, CK 29 novembre 2012
1-1 di 1
Identify P glycoprotein Substrates and Inhibitors with the Rapid HTS Pgp Glo Assay System 4 5 1 1
Hi Dongping Ma and James J. Cali, I'am wondering if you please publish the Pgp ATPase activity EC50s of compounds shown in Figure 4. Thank you. Best Regards, CK 2012/11/29
1-1 / 1
Identify P glycoprotein Substrates and Inhibitors with the Rapid HTS Pgp Glo Assay System 4 5 1 1
Hi Dongping Ma and James J. Cali, I'am wondering if you please publish the Pgp ATPase activity EC50s of compounds shown in Figure 4. Thank you. Best Regards, CK 29 november 2012
1-1 van 1
Identify P glycoprotein Substrates and Inhibitors with the Rapid HTS Pgp Glo Assay System 4 5 1 1
Hi Dongping Ma and James J. Cali, I'am wondering if you please publish the Pgp ATPase activity EC50s of compounds shown in Figure 4. Thank you. Best Regards, CK den 29 november 2012
1-1 av 1
Identify P glycoprotein Substrates and Inhibitors with the Rapid HTS Pgp Glo Assay System 4 5 1 1
Hi Dongping Ma and James J. Cali, I'am wondering if you please publish the Pgp ATPase activity EC50s of compounds shown in Figure 4. Thank you. Best Regards, CK 2012年11月29日
1-1（共 1）

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