GoTaq® Hot Start Polymerase is a new member of the GoTaq® family of products. This enzyme allows hot-start PCR and provides greater specificity, yield and sensitivity. Amplification reactions can be set up at room temperature and put into a room-temperature thermal cycler. In fact, reactions can be assembled and left at room temperature for up to 24 hours before thermal cycling is started. In this article, we describe the properties of GoTaq® Hot Start Polymerase and compare it with standard Taq DNA polymerase. We look at sensitivity using GoTaq® Hot Start Polymerase in both the Green and Colorless GoTaq® Flexi Buffers, and we report on the compatibility of GoTaq® Hot Start Polymerase with RT-PCR. Finally, we compare GoTaq® Hot Start Polymerase with two other commercially available hot-start Taq DNA polymerases.
Promega Notes 99, 8–11.
Kimberly Knoche, Martin Ensenberger and Cynthia Sprecher