DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus Product Information

Literature # 9PIM218

DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is a DNA-dependent DNA polymerase that lacks both the 5´-->´ and 3´-->5´ exonuclease activities present in intact E. coli DNA polymerase I. It is used for random primer labeling and in strand displacement amplification. Klenow Fragment, Exonuclease Minus, will leave a single-base 3´-overhang on a significant proportion of DNA fragments during fill-in of 5´-overhangs. Therefore, this enzyme is not recommended for preparation of blunt-ended fragments for ligation.