pHTN HaloTag® CMV-neo Vector Protocol

Literature # 9PIG772

The pHTN HaloTag® CMV-neo Vector is configured to append the HaloTag® tag to the amino-terminus of the
fused protein. The vector provides constitutive high-level protein expression in mammalian cells using the human cytomegalovirus (CMV) immediate early enhancer/promoter. The vector contains a multiple cloning region for convenient cloning, and can be used for both transient and stable gene expression. The stable expression is mediated by co-expression of the neomycin phosphotransferase gene, which confers resistance to the antibiotic G-418 (Cat.# V7981). Alternatively, the HaloTag® coding region and the accompanying linker can be removed (either using PCR or restriction enzyme digestion) and transferred into other commercially available vectors.