pGEM® Express Positive Control Template Product Information

Literature # 9PIP256

The pGEM® Express Positive Control Template may be used to monitor the performance of the Riboprobe® Systems. Transcription with SP6 RNA Polymerase produces RNA transcripts of 1,787 bases and 2,566 bases. The shorter transcript (1,787 bases) is due to the presence of an SP6 terminator region within the template. The terminator is approximately 50% efficient. Transcription of the template with T7 RNA Polymerase results in transcripts of 1,065 bases and 2,346 bases. The shorter transcript (1,065 bases) is due to the presence of a T7 terminator region within the template. The terminator is approximately 70–80% efficient. Transcription with T3 RNA Polymerase results in transcripts of 250 bases and 1,525 bases. The shorter transcript (250 bases) is again due to the presence of the T7 terminator, which is recognized to some extent by T3 RNA Polymerase.