Anti-ACTIVE® CaM KII pAb, (pT²⁸⁶) and Anti-ACTIVE® Qualified Secondary Antibody...

Literature # TB264

Promega has developed a polyclonal antibody (pAb) to the multifunctional calcium/calmodulin-dependent protein kinase CaM kinase II (CaM KII) that is phosphorylated on threonine 286 (pT²⁸⁶). The Anti-ACTIVE® CaM KII pAb was raised against a phosphothreonine-containing peptide. The recommended dilution of Anti-ACTIVE® CaM KII pAb for Western blot analysis is 1:5,000.

Donkey Anti-Rabbit IgG, (H + L), HRP is an affinity purified-horseradish peroxidase-(HRP) conjugated secondary antibody for use with Anti-ACTIVE® pAbs in Western blot analysis using chemiluminescent and colorimetric detection methods. The antibody conjugate exhibits minimal cross-reactivity to goat, mouse and sheep IgG, bovine serum albumin (BSA) and proteins in mammalian cell extracts. The antibody conjugate provides low backgrounds and highly specific signals when used at the recommended 1:10,000 dilution with Anti-ACTIVE® CaM KII pAb.