CaspACE™ Assay System, Colorimetric Technical Bulletin

Literature # TB270

The CaspACE™ Assay System, Colorimetric, provides reagents to measure the activity of caspase-3, a member of the cysteine aspartic acid-specific protease family. These proteases have been shown to play key roles in apoptosis in mammalian cells. The CaspACE™ Assay System provides a colorimetric substrate and a cell-permeant inhibitor that allow highly sensitive, quantitative measurement of caspase-3 (DEVDase) protease activity, an early regulatory event in the apoptotic cell death process. This assay system may be used with purified enzyme preparations or cell extracts and can be adapted for use in high-throughput systems.