Promega Corporation

Reverse Transcription System Protocol

  • Instructions for Use of Product(s)
  • A3500

Literature # TB099

AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ isolated RNA. The Reverse Transcription System provides lot-tested reagents to efficiently reverse transcribe poly(A)+ mRNA or total RNA in 15 minutes. A polyadenylated 1.2kb transcript is provided as a control template for the cDNA synthesis reaction. cDNA synthesized with the Reverse Transcription System can be used directly in PCR.

Printed in USA. Revised 2/11.

PDF Icon Complete Protocol PDF (111kb)

PDF Icon Quick Protocol PDF (65kb)

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