Serine/Threonine Phosphatase Assay System Technical Bulletin

Literature # TB218

The non-radioactive Serine/Threonine Phosphatase Assay System provides a fast, convenient and flexible method for measuring protein serine/threonine phosphatase activity. This system determines the amount of free phosphate generated in a reaction by measuring the absorbance of a molybdate:malachite green:phosphate complex. The system allows the use of a variety of buffer conditions and substrates, including naturally phosphorylated proteins or synthetic phosphopeptides. In addition to measuring phosphatase activity in partially fractionated and purified samples, the Phosphatase Assay System also can measure phosphatase activity in crude cell or tissue extracts.