TGFß₁ E_max® ImmunoAssay System Technical Bulletin

Literature # TB196

The TGFβ₁ E_max® ImmunoAssay System allows sensitive and specific detection of biologically active TGFβ₁ in an antibody sandwich format. In this format, flat-bottom 96-well plates are coated with TGFβ Coat mAb, which binds soluble TGFβ₁. The captured TGFβ₁ is bound by a second specific polyclonal antibody (pAb). After washing, the amount of specifically bound pAb is detected using a species-specific antibody conjugated to horseradish peroxidase (TGFβ HRP Conjugate) as a tertiary reactant. The unbound conjugate is removed by washing and, following an incubation with a chromogenic substrate, the color change is measured. The amount of TGFβ₁ in the test solutions is proportional to the color generated in the oxidation-reduction reaction. Using this system, biologically active TGFβ₁ in tissue culture supernatants, plasma, serum or urine can be quantitated in the range of 32–1,000pg/ml.

The TGFβ₁ E_max® ImmunoAssay System offers several benefits:

• Specificity: Specific detection of TGFβ₁; typically less than or equal to 3% cross-reactivity with TGFβ₂ and TGFβ₃ at 10ng/ml.


• Sensitivity: Detects a minimum of 32pg/ml of TGFβ₁.


• Flexibility: ELISA plates can be set up in any desired configuration.


• High Value: Optimized reagents and protocol.