Transcend™ Non-Radioactive Translation Detection System Technical Bulletin

Literature # TB182

The Transcend™ Non-Radioactive Translation Detection Systems allow non-radioactive detection of proteins synthesized in vitro. Using these systems, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [³⁵S]methionine or other radioactive amino acids. This biotinylated lysine is added to the translation reaction as a precharged, ε-labeled biotinylated lysine-tRNA complex (Transcend™ tRNA) rather than a free amino acid. After SDS-PAGE and electroblotting, the biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish Peroxidase (Streptavidin-HRP), followed either by colorimetric or chemiluminescent detection. Typically, 0.5–5ng of protein can be detected by these methods within 3–4 hours after gel electrophoresis.