Wizard® Plus Megapreps DNA Purification System Technical Bulletin

Literature # TB140

Large-scale plasmid preparations, such as cesium chloride purification, can be both laborious and time-consuming, often requiring an overnight centrifugation. The Wizard® Plus Megapreps DNA Purification System is simple and rapid and requires only a centrifuge, vacuum source and vacuum manifold such as the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. The system yields up to one milligram of high-copy-number plasmid DNA (200–20,000bp) from a 1,000ml culture in less than three hours.

The Wizard® Plus Megapreps System does not require organic extractions or cesium chloride gradients. Purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) or TE buffer. The purified plasmid can be used directly for DNA sequencing and restriction digestion without further manipulation and can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).