BacTiter-Glo™ Microbial Cell Viability Assay Technical Bulletin

Literature # TB337

The BacTiter-Glo™ Microbial Cell Viability Assay is a homogeneous method to determine the number of viable bacterial cells in culture based on quantitation of the ATP present. ATP is an indicator of metabolically active cells. The BacTiter-Glo™ Assay is designed for either single-tube or multiwell-plate formats for high-throughput screening (HTS). The homogeneous assay procedure involves adding a single reagent (BacTiter-Glo™ Reagent) directly to bacterial cells in medium and measuring luminescence. Washing cells, removing culture medium and performing multiple pipetting steps are not required.