CytoTox-Fluor™ Cytotoxicity Assay Technical Bulletin
Literature # TB350
The CytoTox-Fluor™ Cytotoxicity Assay is a single-reagent-addition, homogeneous, fluorescent assay that measures the relative number of dead cells in cell populations. The CytoTox-Fluor™ Assay measures a distinct protease activity associated with cytotoxicity. The assay uses a fluorogenic peptide substrate (bis-alanyl-alanyl phenylalanyl-rhodamine 110; bis-AAF-R110) to measure dead-cell protease activity, which has been released from cells that have lost membrane integrity. The bis-AAF-R110 Substrate cannot cross the intact membrane of live cells and therefore gives no signal from live cells.
The CytoTox-Fluor™ Assay accommodates downstream multiplexing with many Promega luminescent assays or spectrally distinct fluorescent assay methods, such as assays measuring caspase activation, reporter expression or orthogonal measures of viability.
Printed in USA. Revised 12/12.