DNA IQ™ System—Database Protocol Technical Bulletin

Literature # TB297

The DNA IQ™ System is a DNA isolation and quantitation system designed specifically for the forensic and paternity communities. This system employs a novel technology with magnetic particles to prepare clean samples for short tandem repeat (STR) analysis easily and efficiently. The DNA IQ™ System can be used to extract DNA from stains or liquid samples such as blood or resulting DNA solutions.

The DNA IQ™ resin has a defined DNA-binding capacity in the presence of excess DNA. Yields will be consistent within a single sample type but will differ with different sample types. The typical yield for FTA^® blood card punches is 50–100ng, for liquid blood, 50–200ng; and for buccal swabs, 100–500ng. Since recovery depends on the sampling method, solid support and sample type, laboratories will need to determine the average yield for a single sample type. Once this average yield has been determined, the researcher can bypass the quantitation step typically necessary with other purification procedures.