PDE-Glo™ Phosphodiesterase Assay Technical Bulletin

Literature # TB353

The PDE-Glo™ Phosphodiesterase Assay is a luminescent, high-throughput screening method for measuring cyclic nucleotide phosphodiesterase (PDE) activity from purified sources. PDEs have been implicated in a variety of diseases such as erectile dysfunction, asthma, chronic obstructive pulmonary disease, learning disorders, depression and memory functions, neurodegenerative diseases, autoimmune disorders, heart failure and myocardial infarction. The availability of selective inhibitors for PDEs has facilitated their use as tools to study cyclic nucleotide signaling and paved the way to investigate the role of PDEs in cellular and tissue pathologies. The PDE-Glo™ Phosphodiesterase Assay allows lead candidates for PDE inhibitors to be identified.