T7 RiboMAX™ Express Large Scale RNA Production System Technical Bulletin

Literature # TB298

The T7 RiboMAX™ Express Large Scale RNA Production System is an in vitro transcription system designed for consistent production of milligram amounts of RNA in a short amount of time. This is about 10- to 20-fold more RNA than is produced in a standard Riboprobe® System transcription reaction. T7 RiboMAX™ Express System reactions differ from Riboprobe® System reactions in three primary ways: a HEPES (pH 7.5) buffer is used rather than a Tris-HCl (pH 7.9) buffer, NTP and magnesium concentrations are elevated and inorganic pyrophosphatase is included in the reaction.